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KMID : 1134820070360070813
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 7 p.813 ~ p.818
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Polyphenol Compound Contents and Physiological Activities in Various Extracts of the Vitex rotundifolia Stems
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Joo Eun-Young
Lee Yang-Suk
Kim Nam-Woo
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Abstract
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For this study, extracts of Vitex rotundifolia stems were prepared using reflux water extraction (WE), reflux ethanol extraction (EE) and hot water extract under high pressure (HWE). The extracts were investigated for the total content of phenolic compounds, antioxidant activities, and inhibitory potencies for xanthine oxidase and tyrosinase. The EE extraction method yielded the highest content of polyphenol compounds (176.34 ㎎/g). The electron donating abilities (EDA) were 93.46～96.92%, when extracts were assayed at 1.0 ㎎/mL. The superoxide dismutase (SOD)-like activity was the highest in the WE extract (47.32% at 1.0 ㎎/mL). The nitrite scavenging abilities (pH 1.2) were 84.61～88.36% and the inhibition of xanthine oxidase were over 90% at 0.5 ㎎/mL. Tyrosinase inhibition of HWE and WE were 57.84% and 53.47% respectively. It implies that V. rotundifolia stems have potent physiological activities and their activities were differently exhibited depending on solvent fractions.
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KEYWORD
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Vitex rotundifolia stems, polyphenol, electron donating ability, SOD-like activity, nitrite scavenging ability, xanthine oxidase, tyrosinase
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